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Western blot analysis of GLUT1 protein levels under different experimental conditions. (A) GLUT1 protein expression was significantly reduced in PGAM1-knockdown cell lines and significantly elevated in PGAM1-overexpressing cell lines. (B) GLUT1 protein expression was significantly reduced in METTL5-knockdown cell lines and significantly elevated in METTL5-overexpressing cell lines. (C) GLUT1 protein expression was upregulated in METTL5-silenced cells co-transfected with <t>PGAM1</t> <t>overexpression</t> plasmid. β-actin was used as the loading control. ***P<0.001 and ****P<0.0001. GLUT1, glucose transporter type 1; PGAM1, phosphoglycerate mutase 1; METTL5, methyltransferase 5; NC, negative control; oe, overexpression; si, small interfering RNA.
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Western blot analysis of GLUT1 protein levels under different experimental conditions. (A) GLUT1 protein expression was significantly reduced in PGAM1-knockdown cell lines and significantly elevated in PGAM1-overexpressing cell lines. (B) GLUT1 protein expression was significantly reduced in METTL5-knockdown cell lines and significantly elevated in METTL5-overexpressing cell lines. (C) GLUT1 protein expression was upregulated in METTL5-silenced cells co-transfected with <t>PGAM1</t> <t>overexpression</t> plasmid. β-actin was used as the loading control. ***P<0.001 and ****P<0.0001. GLUT1, glucose transporter type 1; PGAM1, phosphoglycerate mutase 1; METTL5, methyltransferase 5; NC, negative control; oe, overexpression; si, small interfering RNA.
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Western blot analysis of GLUT1 protein levels under different experimental conditions. (A) GLUT1 protein expression was significantly reduced in PGAM1-knockdown cell lines and significantly elevated in PGAM1-overexpressing cell lines. (B) GLUT1 protein expression was significantly reduced in METTL5-knockdown cell lines and significantly elevated in METTL5-overexpressing cell lines. (C) GLUT1 protein expression was upregulated in METTL5-silenced cells co-transfected with PGAM1 overexpression plasmid. β-actin was used as the loading control. ***P<0.001 and ****P<0.0001. GLUT1, glucose transporter type 1; PGAM1, phosphoglycerate mutase 1; METTL5, methyltransferase 5; NC, negative control; oe, overexpression; si, small interfering RNA.

Journal: Oncology Letters

Article Title: METTL5 reprograms glycolytic metabolism and promotes non-small cell lung cancer progression by modifying PGAM1

doi: 10.3892/ol.2026.15609

Figure Lengend Snippet: Western blot analysis of GLUT1 protein levels under different experimental conditions. (A) GLUT1 protein expression was significantly reduced in PGAM1-knockdown cell lines and significantly elevated in PGAM1-overexpressing cell lines. (B) GLUT1 protein expression was significantly reduced in METTL5-knockdown cell lines and significantly elevated in METTL5-overexpressing cell lines. (C) GLUT1 protein expression was upregulated in METTL5-silenced cells co-transfected with PGAM1 overexpression plasmid. β-actin was used as the loading control. ***P<0.001 and ****P<0.0001. GLUT1, glucose transporter type 1; PGAM1, phosphoglycerate mutase 1; METTL5, methyltransferase 5; NC, negative control; oe, overexpression; si, small interfering RNA.

Article Snippet: METTL5- and PGAM1-overexpression plasmids were purchased from Heyuan Liji (Shanghai) Biotechnology Co., Ltd.

Techniques: Western Blot, Expressing, Knockdown, Transfection, Over Expression, Plasmid Preparation, Control, Negative Control, Small Interfering RNA